• Anti-proAKAP4 Monoclonal Antibody
Anti-proAKAP4 Monoclonal Antibody-clone 5H2
Product Specifications
Research Area Reproductive Biology
Purity Mouse monoclonal antibodies 5H2 was purified by protein A/G affinity chromatography. Purity > 90%, as determined by SDS-PAGE and visualized by silver staining.
Host Species Mouse
Reactive Species Goat,Camel,Cat,Dog,Pig,Rabbit,Human
Target proAKAP4
Tested Applications WB,IF,IHC,FCM
Clonality Monoclonal
Isotype IgG2a Kappa
Conjuation Label Unconjugated
Sample Types Semen
Physical Format Liquid Form
Shipping Conditions Room Temperature
Storage Conditions (-)20 deg.C
Clone Name clone 5H2
Storage Buffer PBS
Formulation Solution in PBS at 1 mg/mL
Regulatory Information RUO
Technical Notes WB : 0.1 µg/mL IHC: 5 µg/mL (concentrations may be adjusted according sample types)
Application Notes Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use. This monoclonal antibody (clone 5H2) only recognizes the precursor/proform of AKAP4 (110 kDa / 854 AA), the prodomain (21 kDa) and does not react with the AKAP4 as a result of conversion (82 kDa / 665 AA).
Application ProAKAP4 protein is the precursor of AKAP4 (A-Kinase Anchor Protein 4) that is a structural protein playing a key role in sperm motility, capacitation and fecundation. Sperm without proAKAP4 and AKAP4 are a+B6:T6+I6:T6bnormal, immotile and infertile. Concentrations of proAKAP4 are good indicators of sperm quality and male fertility and indicate if the spermatozoa will stay motile and fertile over time.
Articles & Publications Blommaert D, Franck T, Lejeune JF, Selleslagh M, Jouy N, Sergeant N, Serteyn D and Delehedde M (2019b) The Horse 4MID® Kit as a promising tool to compare different freezing media to preserve stallion semen. Rev. Bras. Reprod. Anim. Vol 43(2):585. Blommaert D, Sergeant N, Delehedde M, Jouy N, Mitchell V, Franck T, Donnay I, Lejeune JP and Serteyn D (2019b) Expression, localization, and concentration of A-kinase anchor protein 4 (AKAP4) and its precursor (proAKAP4) in equine semen: promising marker correlated to the total and progressive motility in thawed spermatozoa. Theriogenology. Vol. 131:52-60. Carracedo S, Loyens A, Eddarkaoui S, Serteyn D, Malo C, Skidmore L, Briand-Amirat L, Barbotin AL, Maurage CA, Delehedde M and Sergeant N (2020) The sperm specific proAKAP4 polypeptide exhibited conserved functions, localizations and metabolism among mammals. Animal Reproduction Science. Vol. 220 :106448 Delehedde M, Carracedo S, Duchene B, Demouveaux B, Remy G, Kervoaze G, Aubry S, Gevaert MH, Maurage CA, Jonckheere N, Van Seuningen I, Philippe Gosset G, Desseyn JL, Sergeant N and Pichavant M (2020) Cigarette smoking affects the sperm-specific proAKAP4 concentrations and impairs both spermatogenesis and sperm quality. Andrology. Vol. 8 (Suppl. 1):45-46. Delehedde M, Carracedo S, Selleslagh M, Eddarkaoui S, Amirat-Briand L and Sergeant N (2019) ProAKAP4 polypeptide as a biomarker of sperm functionality and male fertility disorders. Int J Gynecol and Reprod Sci. Vol. 2(1):13-19. Delehedde M, Demouveaux B, Remy G, Selleslagh M, Dewulf Q, Desseyn JL, Moreau M, Gosset P, Muriel Pichavant M and Sergeant N (2019a) ProAKAP4 concentrations as an indicator of good spermatogenesis and sperm quality under oxidative stress conditions. Andrology. Vol. 7(1):86. Griffin R, Swegen A, Baker M, John Aitken R, A Skerrett-Byrne D, Silva Rodriguez A, E Martín-Cano F, Nixon B, J Peña F, Delehedde M, Sergeant N and Gibb Z (2020) Mass spectrometry reveals distinct proteomic profiles in high- and low-quality stallion spermatozoa. Reproduction. 160(5):695-707. Langlade C, Buff S, Dias C, Commin L (2020) Assessment of Optimized Frozen/Thawed Semen Samples in Canines with the New A-Kinase Anchor Protein 4 Precursor Biomarker. Biopreserv Biobank. 18(5):409-414. Riesco M, Anel-Lopez L, Neila-Montero M, Palacin-Martinez C, Montes-Garrido R, Alvarez M, de Paz P, Anel L (2020) ProAKAP4 as Novel Molecular Marker of Sperm Quality in Ram: An Integrative Study in Fresh, Cooled and Cryopreserved Sperm. Biomolecules. 10(7):1046. doi: 10.3390/biom10071046. Ruelle I, Sergeant N, Bencharif D, Charreaux F, Thorin C, Michaud S, Dordas-Perpinyà M, Jouy N, Audry S, Maurage CA, Delehedde M and Briand-Amirat L (2020) ProAKAP4 concentrations in semen as a predictive tool of Bovine fertility: A preliminary study. Reproduction, Fertility and Development. 32(1):145. Scovell JM, Bournat JC, Szafran AT, Solis M, Moore J, Rivera A, Chen CH, Zhang J, Wilken N, Seth A, Jorgez CJ (2021) PRSS50 is a testis protease responsible for proper sperm tail formation and function. Development Vol.148 (8): dev197558. Sergeant N, Blommaert D, Carracedo S, Franck T, Lejeune JP, Gevaert MH, Aubry S, Maurage CA, Serteyn D and Delehedde M (2020) Assessment of the proAKAP4 concentrations as a pertinent sperm parameter to select extenders and preservatives of stallion semen. Animal Reproduction Science. Vol. 220 :106449. P89. https://www.sciencedirect.com/science/article/abs/pii/S0378432020303213#! Sergeant N, Carracedo S, Blommaert D, Aubry S, Jouy N, Lejeune JP, Franck T, Maurage CA, Serteyn D, Buée L and Delehedde M (2020) Proteolysis of proAKAP4 in semen as a regulatory sensor of sperm quality and functionality. Andrology. Vol. 8 (Suppl. 1):44-45.

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Anti-proAKAP4 Monoclonal Antibody

  • Brand: 4BioDx
  • Product Code: 4BioDx-4BDX-1901S
  • Availability: Pre-Order

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Tags: proAKAP4, sperm, semen