Anti-Tau [pS422] Monoclonal Antibody-clone 2H9

Product Specifications
Research Area	Neuroscience
Purity	Mouse monoclonal antibodies 2H9 was purified by protein A/G affinity chromatography. Purity > 90%, as determined by SDS- PAGE and visualized by Coomassie blue staining.
Host Species	Mouse
Reactive Species	Human,Mouse,Rat
Target	Tau [pS422]
Tested Applications	WB,IF,IHC,FCM
Clonality	Monoclonal
Isotype	IgG2a Kappa
Conjuation Label	Unconjugated
Sample Types	Cells,Tissues
Physical Format	Liquid Form
Shipping Conditions	Room Temperature
Storage Conditions	(-)20 deg.C
Clone Name	clone 2H9
Storage Buffer	PBS
Formulation	Solution in PBS at 1 mg/mL
Regulatory Information	RUO
Technical Notes	WB: 0.2 µg/mL IHC / IF : 2 µg/mL (working with cells, tissue sections (frozen and paraffin-embedded)
Application Notes	Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use.
Application	Tau proteins are encoded by a single gene MAPT (Gene ID: 4137). They belong to the family of microtubule-associated proteins. The phosphorylation of Tau protein at serine 422 is a pathological epitope and a specific marker of neurofibrillary degeneration, one of the neuropathological hallmark of Alzheimer’s disease and also found in more than 20 Tauopathies. On cerebral tissue, frozen or paraffin embedded tissue, 2H9 Tau antibody detects neurons in neurofibrillary degeneration. The serine 422 is phosphorylated by the following kinases: CaMKII (Calcium Calmodulin-kinase II), PKA (protein kinase A), JNKs (c-Jun N-terminal kinases), ERK1/2 or p42/44 MAKP (mitogen-activated kinases), p38 MAPK and MARK (Microtubule-associated protein/microtubule affinity-regulating kinase).

Articles & Publications

Lam S, Petit F, Hérard AS, Boluda S, Eddarkaoui S, Guillermier M, The Brainbank Neuro-CEB Neuropathology Network, Buée L, Duyckaerts C, Haïk S, Picq JL, Dhenain M (2021) Amyloid and tau pathologies, cognitive impairments and cerebral atrophy after Alzheimer brain extracts inoculation in a primate. Acta Neuropathol Commun. in press Lam S, Boluda S, Hérard AS, Petit F, Eddarkaoui S,Cambon K, The Brainbank Neuro-CEB Neuropathology Network, Picq JL, Buee L, Duyckaerts C, Haïk S and Dhenain M (2021) Alzheimer’s brain inoculation in Aß-plaque bearing mice: synaptic loss is linked to tau seeding and low microglial activity. BioRxiv preprint doi: https://doi.org/10.1101/2021.04.06.438654; Tautou M, Eddarkaoui S, Descamps F, Larchanché P-E, El Bakali J, Goveas LM, Dumoulin M, Lamarre C, Blum D, Buée L, Melnyk P and Sergeant N (2021) A ß-Secretase Modulator Decreases Tau Pathology and Preserves Short-Term Memory ina Mouse Model of Neurofibrillary Degeneration. Front. Pharmacol. Vol 12:679335. doi: 10.3389/fphar.2021.679335 Troquier L, Caillierez R, Burnouf S, Fernandez-Gomez FJ, Grosjean ME, Zommer N, Sergeant N, Schraen-Maschke S, Blum D and Buee L (2012) Targeting phospho-Ser422 by active Tau Immunotherapy in the THY-Tau22 mouse model: a suitable therapeutic approach. Curr Alzheimer Res. 9(4):397-405. Sergeant N, Vingtdeux V, Eddarkaoui S, Gay M, Evrard C, Le Fur N, Laurent C, Caillierez R, Obriot H, Larchanché PE, Farce A, Coevoet M, Carato P, Kouach M, Descat A, Dallemagne P, Buée-Scherrer V, Blum D, Hamdane M, Buée L, Melnyk P (2019). New piperazine multi-effect drugs prevent neurofibrillary degeneration and amyloid deposition, and preserve memory in animal models of Alzheimer's disease. Neurobiol Dis. Vol. 129:217-233. Sergeant N, Bretteville A, Hamdane M, Caillet-Boudin ML, Grognet P, Bombois S, Blum D, Delacourte A, Pasquier F, Vanmechelen E , Schraen-Maschke S, and Buee L (2008) Biochemistry of Tau in Alzheimer’s disease and related neurological disorders. Expert review of proteomics 5:207–224.

Anti-Tau [pS422] Monoclonal Antibody

Brand: 4BioDx
Product Code: 4BioDx-4BDX-1501
Availability: Pre-Order

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Tags: Tau, Amyloid