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  • Anti-AKAP4 Monoclonal Antibody
Anti-AKAP4 Monoclonal Antibody-clone 10F8
Product Specifications
Research Area Reproductive Biology
Purity Mouse monoclonal antibodies 10F8 was purified by protein A/G affinity chromatography. Purity > 90%, as determined by SDS-PAGE and visualized by silver staining.
Host Species Mouse
Reactive Species Dog,Rabbit,Pig,Ram,Goat
Target AKAP4
Tested Applications WB,IF,IHC,FCM
Clonality Monoclonal
Isotype IgG2a Kappa
Conjuation Label Unconjugated
Sample Types Semen
Physical Format Liquid Form
Shipping Conditions Room Temperature
Storage Conditions (-)20 deg.C
Clone Name clone 10F8
Storage Buffer PBS
Formulation Solution in PBS at 1 mg/mL
Regulatory Information RUO
Technical Notes WB : 0.1 µg/mL IHC: 5 µg/mL (concentrations may be adjusted according sample types)
Application Notes Research Use Only Product (RUO) / Not for Diagnosis or Therapeutic Use. The monoclonal antibody (clone 10F8) recognizes both the pro-form of AKAP4 (110 kDa / 854 AA) and the AKAP4 (82 kDa / 665 AA) but does not react with the prodomain (21 kDa).
Application AKAP4 (A-Kinase Anchor Protein 4) protein is encoded by a single gene located on chromosome X. The proAKAP4 polypeptide is converted into mature AKAP4 by proteolytic cleavage of the amino-terminal prodomain. AKAP4 and its precursor proAKAP4 are both major components of the pig, horse, bull, mouse, rat, ram, dog, rabbit and human sperm fibrous sheath of the sperm flagellum. AKAP4 protein belongs to the family of A-kinase anchor proteins (AKAPs) all sharing a common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the PKA holoenzyme to discrete locations within the cell. AKAP4 is also named AKAP-4, AKAP82 (A-Kinase Anchor Protein 82 KDa), PRKA4 (Protein Kinase Anchoring Protein 4), HI, CT99 (Cancer/Testis Antigen 99), FSC1 (Fibrous sheath component 1) or P82. AKAP4 plays a major role in flagellum formation, sperm motility, capacitation and fecundation.
Articles & Publications Blommaert D, Franck T, Lejeune JF, Selleslagh M, Jouy N, Sergeant N, Serteyn D and Delehedde M (2019b) The Horse 4MID® Kit as a promising tool to compare different freezing media to preserve stallion semen. Rev. Bras. Reprod. Anim. Vol 43(2):585. Blommaert D, Sergeant N, Delehedde M, Jouy N, Mitchell V, Franck T, Donnay I, Lejeune JP and Serteyn D (2019b) Expression, localization, and concentration of A-kinase anchor protein 4 (AKAP4) and its precursor (proAKAP4) in equine semen: promising marker correlated to the total and progressive motility in thawed spermatozoa. Theriogenology. Vol. 131:52-60. Carracedo S, Loyens A, Eddarkaoui S, Serteyn D, Malo C, Skidmore L, Briand-Amirat L, Barbotin AL, Maurage CA, Delehedde M and Sergeant N (2020) The sperm specific proAKAP4 polypeptide exhibited conserved functions, localizations and metabolism among mammals. Animal Reproduction Science. Vol. 220 :106448 Delehedde M, Carracedo S, Duchene B, Demouveaux B, Remy G, Kervoaze G, Aubry S, Gevaert MH, Maurage CA, Jonckheere N, Van Seuningen I, Philippe Gosset G, Desseyn JL, Sergeant N and Pichavant M (2020) Cigarette smoking affects the sperm-specific proAKAP4 concentrations and impairs both spermatogenesis and sperm quality. Andrology. Vol. 8 (Suppl. 1):45-46. Delehedde M, Carracedo S, Selleslagh M, Eddarkaoui S, Amirat-Briand L and Sergeant N (2019) ProAKAP4 polypeptide as a biomarker of sperm functionality and male fertility disorders. Int J Gynecol and Reprod Sci. Vol. 2(1):13-19. Delehedde M, Demouveaux B, Remy G, Selleslagh M, Dewulf Q, Desseyn JL, Moreau M, Gosset P, Muriel Pichavant M and Sergeant N (2019a) ProAKAP4 concentrations as an indicator of good spermatogenesis and sperm quality under oxidative stress conditions. Andrology. Vol. 7(1):86. Griffin R, Swegen A, Baker M, John Aitken R, A Skerrett-Byrne D, Silva Rodriguez A, E Martín-Cano F, Nixon B, J Peña F, Delehedde M, Sergeant N and Gibb Z (2020) Mass spectrometry reveals distinct proteomic profiles in high- and low-quality stallion spermatozoa. Reproduction. 160(5):695-707. Langlade C, Buff S, Dias C, Commin L (2020) Assessment of Optimized Frozen/Thawed Semen Samples in Canines with the New A-Kinase Anchor Protein 4 Precursor Biomarker. Biopreserv Biobank. 18(5):409-414. Riesco M, Anel-Lopez L, Neila-Montero M, Palacin-Martinez C, Montes-Garrido R, Alvarez M, de Paz P, Anel L (2020) ProAKAP4 as Novel Molecular Marker of Sperm Quality in Ram: An Integrative Study in Fresh, Cooled and Cryopreserved Sperm. Biomolecules. 10(7):1046. doi: 10.3390/biom10071046. Ruelle I, Sergeant N, Bencharif D, Charreaux F, Thorin C, Michaud S, Dordas-Perpinyà M, Jouy N, Audry S, Maurage CA, Delehedde M and Briand-Amirat L (2020) ProAKAP4 concentrations in semen as a predictive tool of bull fertility: A preliminary study. Reproduction, Fertility and Development. 32(1):145. Scovell JM, Bournat JC, Szafran AT, Solis M, Moore J, Rivera A, Chen CH, Zhang J, Wilken N, Seth A, Jorgez CJ (2021) PRSS50 is a testis protease responsible for proper sperm tail formation and function. Development Vol.148 (8): dev197558. Sergeant N, Blommaert D, Carracedo S, Franck T, Lejeune JP, Gevaert MH, Aubry S, Maurage CA, Serteyn D and Delehedde M (2020) Assessment of the proAKAP4 concentrations as a pertinent sperm parameter to select extenders and preservatives of stallion semen. Animal Reproduction Science. Vol. 220 :106449. P89. https://www.sciencedirect.com/science/article/abs/pii/S0378432020303213#! Sergeant N, Carracedo S, Blommaert D, Aubry S, Jouy N, Lejeune JP, Franck T, Maurage CA, Serteyn D, Buée L and Delehedde M (2020) Proteolysis of proAKAP4 in semen as a regulatory sensor of sperm quality and functionality. Andrology. Vol. 8 (Suppl. 1):44-45.

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Anti-AKAP4 Monoclonal Antibody

  • Brand: 4BioDx
  • Product Code: 4BioDx-4BDX-1805S
  • Availability: Pre-Order

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Tags: AKAP4, sperm, semen