The NT-proANP Sandwich ELISA kit is a test that is intended for the quantitative measurement of N-terminal atrial natriuretic peptide levels in human, dog, rat, or mouse serum, EDTA plasma, heparin plasma, cell culture supernatant and urine samples.

The NT-proANP ELISA is an independently validated test with >170 NT-proANP ELISA specific publications to date.

The NT-proANP ELISA was also successfully used in pre-clinical rat studies of pharmaceutical companies due to the highly conserved sequence between human and rat NT-proANP. Target Information: Atrial natriuretic peptide is synthesized in atrial myocytes and is stored in secretory granules as a 126 amino acid prohormone. The most important stimulus for the release of the hormone into circulation is stretch of the myocyte fibres. On release the prohormone is split into equimolar amounts of the highly biologically active proANP (99-126), also known as α-ANP, and the N-terminal part proANP (1-98). α-ANP is rapidly cleared from the circulation with a half-life of 3-4 minutes. NT-proANP (1-98) has a much longer half-life (60-120 min) which leads to significantly higher concentrations in blood compared to α-ANP. Thus, circulating levels of NT-proANP (1-98) are less sensitive to the pulsatile secretion of ANP and may better reflect chronic levels than the rapidly fluctuating levels of α-ANP. NT-proANP is discussed as valuable marker for e.g. sepsis (Increased plasma levels of NT-proANP and NT-proBNP as markers of cardiac dysfunction in septic patients.), or risk stratification in heart failure (Neurohormonal risk stratification for sudden death and death owing to progressive heart failure in chronic heart failure).