The Horse 4MID® KIT is the first ELISA assay that objectively validates semen quality by quantifying a protein biomarker called proAKAP4 in sperm samples (in ejaculate, chilled or frozen semen in extender or in isolated spermatozoa).
| Product Specifications |
| Research Area |
Reproductive Biology |
| Assay Type |
ELISA Kit |
| Reactive Species |
Horse |
| Target |
Horse proAKAP4 |
| Tested Applications |
ELISA |
| Sample Types |
Semen |
| Physical Format |
12 breakable Strips of 8-wells |
| Shipping Conditions |
Room Temperature |
| Storage Conditions |
4 deg. C |
| Sensitivity |
4 ng/mL |
| Assay Time |
2h to 3h |
| Detection Method |
ELISA |
| Assay Range |
0 to 150 ng |
| Materials Required (Not Provided) |
- 96-well microplate reader measuring absorbance at 450 nm
- Horizontal orbital microplate shaker (300 rpm)
- Vortex
- Multichannel pipette of 300 μL
- Pipettes of 20 μL, 200 μL and 1000 μL
- Pipette tips
- Polypropylene microtubes of 1.5 mL (for sample preparation)
- One polypropylene tube of 15 mL (for R6 dilution)
- Plastic reagent reservoirs
- One glass bottle (for R2 dilution)
- Scissors
- Pen
- Aluminum foil
- Ultrapure or double deionized water |
| Product Contents |
R1 - Capture Antibody Coated Microplate of 96-wells (12 x 8-well strips) R2 - 1 Bottle of 10x Washing Buffer Solution (30 mL)
R3 - 1 Bottle of 1x Dilution Buffer (30 mL)
R4 - 7 Vials of Ready-to-Use Standard Solution (0.7 mL per vial)
R5 - 1 Bottle of 1x Horse Spermatozoa Lysis Buffer (30 mL)
R6 - 1 Vial of Detection Antibody (0.1 mL)
R7 - 1 Bottle of Substrate Solution (11 mL)
R8 - 1 Bottle 1x Stop Solution (6 mL)
R9 - 1 Vial of Positive Control (0.7 mL) R10 - 2 Adhesive Plate Sealers
R11 - User Manual Instructions |
| Regulatory Information |
RUO |
| Technical Notes |
From one to 88 samples can be run simultaneously or individually (12 breakable strips of 8 wells per 96 well microplate / per kit) |
| Application |
Testing Sperm Vitality in samples such as Ejaculates, semen in extenders and Isolated spermatozoa |
| Articles & Publications |
First results about ProAKAP4 concentration in stallion semen after cryopreservation in two different freezing media. Cryobiology. Vol. 102:133-135. 10.1016/j.cryobiol.2021.07.009 | Mass spectrometry reveals distinct proteomic profiles in high- and low-quality stallion spermatozoa. Reproduction. 160(5):695-707 | Significant correlation between the proAKAP4 concentration and the total and progressive motility in stallion sperm after thawing. Journal of Equine Veterinary Science. Vol. 66:43. |
