ENDOTOXIN LEVEL <0.1 EU per 1 μg of the protein by the LAL method. Human Recombinant GMP Grade IL-1 beta (Interleukin-1 beta). Interleukin-1 beta (IL-1β) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a cytokine protein that in humans is encoded by the IL1B gene. There are two genes for interleukin-1 (IL-1): IL-1 alpha and IL-1 beta (this gene). IL-1β precursor is cleaved by cytosolic caspase 1 (interleukin-1 beta convertase) to form mature IL-1β.
| Product Specifications | |
| Research Area | 2D-3D Cell Culture,Cell Therapy |
| Purity | >98% by SDS-PAGE |
| Target | IL-1b |
| Tested Applications | Cell Culture,Cell Therapy |
| Conjuation Label | His-Tag |
| Grade | GMP Grade |
| Physical Format | Lyophilized |
| Animal Free | Yes |
| Source(Natural/Synthetic) | Recombinant |
| Origin | E.coli |
| Sterile | Sterile |
| Animal Model | Human |
| Shipping Conditions | Blue Ice |
| Storage Conditions | Store at -20degC |
| Storage Buffer | 1X PBS-pH8.0 |
| UniProtKB | P01584 |
| Protein Type | Bioactive |
| Technical Notes | Protein Sequence - MASAPVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDP KNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS with polyhistidine tag at the C-terminus |
| Application Notes | BIOACTIVITY-Measure by its ability to induce proliferation in D10.G4.1 cells. The ED50 for this effect is <10 pg/mL. The specific activity of recombinant human IL-1 beta is approximately >1.5 x 108 IU/mg. Measure by its ability to induce IL-8 secretion in HT29 cells. The ED50 for this effect is <2.0 ng/mL. |
Human IL-1 beta (Interleukin-1 beta) His-Tag GMP Grade Recombinant Protein
- Brand: Croyez
- Product Code: Croyez-C01002-GMP-100
- Availability: Pre-Order