ENDOTOXIN LEVEL <0.1 EU per 1 μg of the protein by the LAL method. Human Recombinant GMP Grade TNF alpha (Tumor necrosis factor alpha). Tumor necrosis factor alpha (TNF alpha) is a kind of pleiotropic pro-inflammatory cytokine. It is secreted by various cells, such as adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. Proteolysis of the integral membrane precursor form of TNF alpha from cells soluble can release homotrimeric TNF alpha. TNF alpha can bind with some TNF alpha receptors induces apoptosis, besides, also trigger other responses depending on cell type, receptor expression, and signal transduction status. TNF alpha participate in the inflammatory response.