The human Big Endothelin-1 Sandwich ELISA kit is a reliable day test that is intended for the quantitative measurement of Big Endothelin-1 levels in human serum, EDTA plasma, heparin and citrate plasma samples.

The Big Endothelin-1 ELISA is a highly recognized test with over 80 specific publications to date.

Target Information: Cleavage of Big Endothelin-1 by a membrane-bound metalloproteinase, the Endothelin Converting Enzyme (ECE), leads to the active ET (1-21), a potent vasoconstrictor, and to the biological inactive C-terminal fragment (22-38).

The half-life of ET-1 in plasma is less than one minute, whereas clearance of Big ET-1 is much slower. Endothelin-1 has been identified in a variety of tissues, including lung, kidney, brain, pituitary and peripheral endocrine tissues, and placenta. The biological role of ET-1 extends beyond regulating vascular tone also in its growth regulatory properties. The peptide interacts in an autocrine/paracrine manner with specific ET receptors found on numerous cells, including smooth muscle cells, myocytes, and fibroblasts.