Anti-APP phosphorylated at threonine 668-Polyclonal Antibody
| Product Specifications |
| Research Area |
Neuroscience |
| Host Species |
Rabbit |
| Reactive Species |
Human,Mouse,Rat |
| Target |
APP [pT668] |
| Tested Applications |
WB,IHC,IF |
| Clonality |
Polyclonal |
| Conjuation Label |
Unconjugated |
| Sample Types |
Cells,Tissues |
| Physical Format |
Liquid Form |
| Shipping Conditions |
Room Temperature |
| Storage Conditions |
(-)20 deg.C |
| Storage Buffer |
Glycerol |
| Formulation |
Rabbit serum diluted in glycerol with 0.05% sodium azide. |
| Regulatory Information |
RUO |
| Technical Notes |
WB : 1:4000 in Tris HCl pH 8.0, Tween 20 0.05% and 5% dry skimmed milk (working with cells and tissues)
IHC: 1:200 in PBS with 5% BSA (working with cells, tissue sections (frozen and parrafin embedded)
(concentrations may be adjusted according sample types) |
| Application Notes |
Obtained by immunizing a rabbit with a 12 amino acid peptide containing the phosphotylated threonine 668 (YVDAAVTPEERH). The phosphorylated threonine is underlined. |
| Application |
Amyloid protein precursor also called APP (Gene ID: 351) is a type 1 transmembrane protein whose metabolism leads to the production of amyloid-beta peptides. In Alzheimer’s disease, amyloid peptides are the main components of amyloid deposits. Phosphorylation of APP at threonine 668 is increased in Alzheimer’s disease and promotes amyloid-beta peptide production. The threonine 668 is phosphorylated by CDK5 (cyclin dependent kinase 2), JNKs (c-Jun N-terminal kinases) and GSK3 (glycogen synthase kinase 3) and DYRK1A (Dual-specificity tyrosine(Y)-phosphorylation regulated kinase 1A). |
| Articles & Publications |
Vingtdeux V, Hamdane M, Gompel M, Begard S, Drobecq H, Ghestem A, Grosjean ME, Kostanjevecki V, Grognet P, Vanmechelen E, Buee L, Delacourte A, Sergeant N (2005). Phosphorylation of amyloid precursor carboxy-terminal fragments enhances their processing by a gamma-secretase-dependent mechanism. Neurobiol Dis. 20(2): 625-637. |
 Antibody](https://4biodx.com/54/anti-app-pt668-80ul.jpg "Anti-APP [pT668](polyclonal) Antibody")