The ProAKAP4 ELISA Kit is the first ELISA assay that objectively validates semen quality by quantifying a protein biomarker called proAKAP4 in sperm samples (in ejaculate, chilled or frozen semen in extender or in isolated spermatozoa).
| Product Specifications | |
| Research Area | Reproductive Biology |
| Reactive Species | Rodents,Human |
| Target | proAKAP4 domain of mouse, rat and human spermatozoa |
| Tested Applications | ELISA |
| Sample Types | Semen |
| Assay Type | Quantitative Assay |
| Physical Format | 12 breakable Strips of 8-wells |
| Shipping Conditions | Room Temperature |
| Storage Conditions | 4 deg. C |
| Sensitivity | 4 ng/mL |
| Assay Time | 2h to 3h |
| Detection Method | ELISA |
| Assay Range | 0 to 150 ng |
| Materials Required (Not Provided) | - 96-well microplate reader measuring absorbance at 450 nm - Horizontal orbital microplate shaker (300 rpm) - Vortex - Multichannel pipette of 300 μL - Pipettes of 20 μL, 200 μL and 1000 μL - Pipette tips - Polypropylene microtubes of 1.5 mL (for sample preparation) - One polypropylene tube of 15 mL (for R6 dilution) - Plastic reagent reservoirs - One glass bottle (for R2 dilution) - Scissors - Pen - Aluminum foil - Ultrapure or double deionized water |
| Product Contents | R1 - Capture Antibody Coated Microplate of 96-wells (12 x 8-well strips) R2 - 1 Bottle of 10x Washing Buffer Solution (30 mL) R3 - 1 Bottle of 1x Dilution Buffer (30 mL) R4 - 7 Vials of Ready-to-Use Standard Solution (0.7 mL per vial) R5 - 1 Bottle of 1x Spermatozoa Lysis Buffer (30 mL) R6 - 1 Vial of Detection Antibody (0.1 mL) R7 - 1 Bottle of Substrate Solution (11 mL) R8 - 1 Bottle 1x Stop Solution (6 mL) R9 - 1 Vial of Positive Control (0.7 mL) R10 - 2 Adhesive Plate Sealers R11 - User Manual Instructions |
| Regulatory Information | RUO |
| Technical Notes | From one to 88 samples can be run simultaneously or individually (12 breakable strips of 8 wells per 96 well microplate / per kit) |
| Application | Testing Sperm Vitality in samples such as Ejaculates, semen in extenders and Isolated spermatozoa |
| Articles & Publications | Carracedo S, Briand-Amirat L, Dordas-Perpinyà M, Ramos Escuredo Y, Delcombel R, Sergeant N and Dele-hedde M (2022) ProAKAP4 protein marker: towards a functional approach to male fertility. Animal Reproduc-tion Science (in Press). Delehedde M, Demouveaux B, Remy G, Selleslagh M, Dewulf Q, Desseyn JL, Moreau M, Gosset P, Muriel Pichavant M and Sergeant N (2019) ProAKAP4 concentrations as an indicator of good spermatogenesis and sperm quality under oxidative stress conditions. Andrology. Vol. 7(1):86. Delehedde M, Carracedo S, Selleslagh M, et al. ProAKAP4 polypeptide as a biomarker of sperm functionality and male fertility disorders. Int J Gynecol and Reprod Sci. (2019);2(1):13-19 Escalier D and Albert M (2006) New fibrous sheath anomaly in spermatozoa of men with consanguinity. Fertility and Sterility. Vol. 86:1-9. Jumeau F, Sigala J, Dossou-Gbete F, Frimat K, Barbotin AL, Buée L, Béhal H, Sergeant N and Mitchell V (2018) A-kinase anchor protein 4 precursors (pro-AKAP4) in human sperm. Andrology. Vol. (6):854-859. Jumeau F, Sigala J, Fernandez-Gomez FJ, Eddarkaoui S, Duban-Deweer S, Buée L, Béhal H, Sergeant N and Mitchell V (2018) Gel electrophoresis of human sperm: a simple method for evaluating sperm protein quality. Basic Clinical Andrology. Vol. (28)10:1-8. Luconi M, Cantini G, Baldi E and Forti G (2011) Role of A-kinase anchoring proteins (AKAPs) in reproduction. Frontiers in Bioscience. Vol. 16:1315-1330. Sergeant N, Briand-Amirat L, Bencharif D and Delehedde M (2019) The sperm specific protein proAKAP4 as an innovative marker to evaluate sperm quality and fertility. Journal of Dairy & Veterinary Sciences. Vol. 11:01-19. Brown PR, Miki K, Harper D and Eddy EM (2003) A-kinase anchoring protein 4 binding proteins in the fibrous sheath. Biology of Reproduction. Vol. 68:2241-2248. Miki K, Goulding EH, Fulcher KD, Eddy E, Willis WD and Brown PR (2002) Targeted disruption of the AKAP4 gene causes defects in sperm flagellum and motility. Developmental Biology. Vol. 248:331-342. |
ProAKAP4 ELISA Kit (Rodent and Human)
- Brand: 4BioDx
- Product Code: 4BioDx-4BDX-18K12RUO
- Availability: Pre-Order