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  • Camel Sperm Vitality Assay Kit (ELISA)
The Camel 4MID® KIT is the first ELISA assay that objectively validates semen quality by quantifying a protein biomarker called proAKAP4 in sperm samples (in ejaculate, chilled or frozen semen in extender or in isolated spermatozoa).
Product Specifications
Research Area Reproductive Biology
Reactive Species Camel
Target Camel proAKAP4
Tested Applications ELISA
Sample Types Semen
Assay Type Quantitative Assay
Physical Format 12 breakable Strips of 8-wells
Shipping Conditions Room Temperature
Storage Conditions 4 deg. C
Sensitivity 4 ng/mL
Assay Time 2h to 3h
Detection Method ELISA
Assay Range 0 to 150 ng
Materials Required (Not Provided) - 96-well microplate reader measuring absorbance at 450 nm - Horizontal orbital microplate shaker (300 rpm) - Vortex - Multichannel pipette of 300 μL - Pipettes of 20 μL, 200 μL and 1000 μL - Pipette tips - Polypropylene microtubes of 1.5 mL (for sample preparation) - One polypropylene tube of 15 mL (for R6 dilution) - Plastic reagent reservoirs - One glass bottle (for R2 dilution) - Scissors - Pen - Aluminum foil - Ultrapure or double deionized water
Product Contents R1 - Capture Antibody Coated Microplate of 96-wells (12 x 8-well strips) R2 - 1 Bottle of 10x Washing Buffer Solution (30 mL) R3 - 1 Bottle of 1x Dilution Buffer (30 mL) R4 - 7 Vials of Ready-to-Use Standard Solution (0.7 mL per vial) R5 - 1 Bottle of 1x Camel Spermatozoa Lysis Buffer (30 mL) R6 - 1 Vial of Detection Antibody (0.1 mL) R7 - 1 Bottle of Substrate Solution (11 mL) R8 - 1 Bottle 1x Stop Solution (6 mL) R9 - 1 Vial of Positive Control (0.7 mL) R10 - 2 Adhesive Plate Sealers R11 - User Manual Instructions
Regulatory Information RUO
Technical Notes From one to 88 samples can be run simultaneously or individually (12 breakable strips of 8 wells per 96 well microplate / per kit)
Application Testing Sperm Vitality in samples such as Ejaculates, semen in extenders and Isolated spermatozoa
Articles & Publications Carracedo S, Briand-Amirat L, Dordas-Perpinyà M, Ramos Escuredo Y, Delcombel R, Sergeant N and Dele-hedde M (2022) ProAKAP4 protein marker: towards a functional approach to male fertility. Animal Reproduc-tion Science (in Press). Malo C, Carracedo S, Delehedde M, Sergeant N and Skidmore JA (2021) Identification of PROAKAP4 in Dromedary sperm and their correlation with monthly semen parameters. Reproduction & Fertility. Vol.2(4):268-279. Carracedo S, Loyens A, Eddarkaoui S, Serteyn D, Malo C, Skidmore L, Briand-Amirat L, Barbotin AL, Maurage CA, Delehedde M and Sergeant N (2020) The sperm specific proAKAP4 polypeptide exhibited conserved functions, localizations and metabolism among mammals. Animal Reproduction Science. Vol. 220 :106448 Dewulf Q, Briand-Amirat L, Eddarkaoui S, Chambonnet F, Delehedde M and Sergeant N (2019) The effects of freeze-thaw cycles and of storage time on the stability of proAKAP4 polypeptide in raw sperm samples: implications for semen analysis assessment in breeding activities. Journal of Dairy & Veterinary Sciences. Vol. 13(3):1-7. Delehedde M, Blommaert D, Jouy N, Scabello J, Miersman H, Franck T, Serteyn D, Mitchell V, and Sergeant S (2018) Concentration of proAKAP4 as a pertinent read-out of sperm quality in mammals. Animal Reproduction Science. Vol. 194:24. Lv C, Larbi A, Memon S, Liang J, Zhao X, Shao Q, Wu G and Quan G (2020) The proteomic characterization of Camel sperm during cryopreservation analyzed by the two-dimensional electrophoresis coupled with mass spectrometry. Cryobiology. 14:S0011-2240(20)30189-9. Riesco M, Anel-Lopez L, Neila-Montero M, Palacin-Martinez C, Montes-Garrido R, Alvarez M, de Paz P, Anel L (2020) ProAKAP4 as Novel Molecular Marker of Sperm Quality in Camel: An Integrative Study in Fresh, Cooled and Cryopreserved Sperm. Biomolecules. 10(7):1046. doi: 10.3390/biom10071046. Sergeant N, Jumeau F, Eddarkaoui S, Sigala J, Dossou GF, Delehedde M, Buee L, Yvoz JF and Mitchell V (2016) Investigating proteomic methods and tools to assess sperm quality. Animal Reproduction Science. Vol. 169:99-135. Sergeant N, Briand-Amirat L, Bencharif D and Delehedde M (2019) The sperm specific protein proAKAP4 as an innovative marker to evaluate sperm quality and fertility. Journal of Dairy & Veterinary Sciences. Vol. 11:01-19. Sergeant N, Carracedo S, Blommaert D, Aubry S, Jouy N, Lejeune JP, Franck T, Maurage CA, Serteyn D, Buée L and Delehedde M (2020) Proteolysis of proAKAP4 in semen as a regulatory sensor of sperm quality and functionality. Andrology. Vol. 8 (Suppl. 1):44-45.

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Camel Sperm Vitality Assay Kit (ELISA)

  • Brand: 4BioDx
  • Product Code: 4BioDx-4VDX-19K11
  • Availability: Pre-Order

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Tags: proAKAP4, sperm, semen