The Goat 4MID® KIT is the first ELISA assay that objectively validates semen quality by quantifying a protein biomarker called proAKAP4 in sperm samples (in ejaculate, chilled or frozen semen in extender or in isolated spermatozoa).
| Product Specifications | |
| Research Area | Reproductive Biology |
| Reactive Species | Goat |
| Target | Goat proAKAP4 |
| Tested Applications | ELISA |
| Sample Types | Semen |
| Assay Type | Quantitative Assay |
| Physical Format | 12 breakable Strips of 8-wells |
| Shipping Conditions | Room Temperature |
| Storage Conditions | 4 deg. C |
| Sensitivity | 4 ng/mL |
| Assay Time | 2h to 3h |
| Detection Method | ELISA |
| Assay Range | 0 to 150 ng |
| Materials Required (Not Provided) | - 96-well microplate reader measuring absorbance at 450 nm - Horizontal orbital microplate shaker (300 rpm) - Vortex - Multichannel pipette of 300 μL - Pipettes of 20 μL, 200 μL and 1000 μL - Pipette tips - Polypropylene microtubes of 1.5 mL (for sample preparation) - One polypropylene tube of 15 mL (for R6 dilution) - Plastic reagent reservoirs - One glass bottle (for R2 dilution) - Scissors - Pen - Aluminum foil - Ultrapure or double deionized water |
| Product Contents | R1 - Capture Antibody Coated Microplate of 96-wells (12 x 8-well strips) R2 - 1 Bottle of 10x Washing Buffer Solution (30 mL) R3 - 1 Bottle of 1x Dilution Buffer (30 mL) R4 - 7 Vials of Ready-to-Use Standard Solution (0.7 mL per vial) R5 - 1 Bottle of 1x Goat Spermatozoa Lysis Buffer (30 mL) R6 - 1 Vial of Detection Antibody (0.1 mL) R7 - 1 Bottle of Substrate Solution (11 mL) R8 - 1 Bottle 1x Stop Solution (6 mL) R9 - 1 Vial of Positive Control (0.7 mL) R10 - 2 Adhesive Plate Sealers R11 - User Manual Instructions |
| Regulatory Information | RUO |
| Technical Notes | From one to 88 samples can be run simultaneously or individually (12 breakable strips of 8 wells per 96 well microplate / per kit) |
| Application | Testing Sperm Vitality in samples such as Ejaculates, semen in extenders and Isolated spermatozoa |
| Articles & Publications | Carracedo S, Briand-Amirat L, Dordas-Perpinyà M, Ramos Escuredo Y, Delcombel R, Sergeant N and Dele-hedde M (2022) ProAKAP4 protein marker: towards a functional approach to male fertility. Animal Reproduc-tion Science (in Press). Fatet A, Sergeant N, Dordas-Perpinyà M, Drouet B, Ponthoreau O, Carracedo Vicente S, Bruyas JF, Thorin C, Delehedde M and Briand-Amirat L (2022) The sperm specific protein proAKAP4 as a marker to evaluate sperm quality and fertility in Bucks. Reproduction in Domestic Animals. Carracedo S, Loyens A, Eddarkaoui S, Serteyn D, Malo C, Skidmore L, Briand-Amirat L, Barbotin AL, Maurage CA, Delehedde M and Sergeant N (2020) The sperm specific proAKAP4 polypeptide exhibited conserved functions, localizations and metabolism among mammals. Animal Reproduction Science. Vol. 220 :106448 - P88 https://www.sciencedirect.com/science/article/abs/pii/S0378432020303201 Sergeant N, Briand-Amirat L, Bencharif D and Delehedde M (2019) The sperm specific protein proAKAP4 as an innovative marker to evaluate sperm quality and fertility. Journal of Dairy & Veterinary Sciences. Vol. 11:01-19 Fang X, Huang LL, Xu J, Ma CQ, Chen ZH, Zhang Z, Liao CH, Zheng SX, Huang P, Xu WM, Li N, Sun L (2019) Proteomics and single-cell RNA analysis of Akap4-knockout mice model confirm indispensable role of Akap4 in spermatogenesis. Developmental Biology. 454(2):118-127. Delehedde M, Blommaert D, Jouy N, Scabello J, Miersman H, Franck T, Serteyn D, Mitchell V, and Sergeant S (2018) Concentration of proAKAP4 as a pertinent read-out of sperm quality in mammals. Animal Reproduction Science. Vol. 194:24. Sergeant N, Jumeau F, Eddarkaoui S, Sigala J, Dossou GF, Delehedde M, Buee L, Yvoz JF and Mitchell V (2016) Investigating proteomic methods and tools to assess sperm quality. Animal Reproduction Science. Vol. 169:99-135. |
Goat Sperm Vitality Assay Kit (ELISA)
- Brand: 4BioDx
- Product Code: 4BioDx-4VDX-18K9
- Availability: Pre-Order