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  • Bull Sperm Vitality Assay Kit (ELISA)
The Bull 4MID® KIT is the first ELISA assay that objectively validates semen quality by quantifying a protein biomarker called proAKAP4 in sperm samples (in ejaculate, chilled or frozen semen in extender or in isolated spermatozoa).
Product Specifications
Research Area Reproductive Biology
Reactive Species Bull
Target Bovine proAKAP4
Tested Applications ELISA
Sample Types Semen
Assay Type Quantitative Assay
Physical Format 12 breakable Strips of 8-wells
Shipping Conditions Room Temperature
Storage Conditions 4 deg. C
Sensitivity 4 ng/mL
Assay Time 2h to 3h
Detection Method ELISA
Assay Range 0 to 150 ng
Materials Required (Not Provided) - 96-well microplate reader measuring absorbance at 450 nm - Horizontal orbital microplate shaker (300 rpm) - Vortex - Multichannel pipette of 300 μL - Pipettes of 20 μL, 200 μL and 1000 μL - Pipette tips - Polypropylene microtubes of 1.5 mL (for sample preparation) - One polypropylene tube of 15 mL (for R6 dilution) - Plastic reagent reservoirs - One glass bottle (for R2 dilution) - Scissors - Pen - Aluminum foil - Ultrapure or double deionized water
Product Contents R1 - Capture Antibody Coated Microplate of 96-wells (12 x 8-well strips) R2 - 1 Bottle of 10x Washing Buffer Solution (30 mL) R3 - 1 Bottle of 1x Dilution Buffer (30 mL) R4 - 7 Vials of Ready-to-Use Standard Solution (0.7 mL per vial) R5 - 1 Bottle of 1x Bull Spermatozoa Lysis Buffer (30 mL) R6 - 1 Vial of Detection Antibody (0.1 mL) R7 - 1 Bottle of Substrate Solution (11 mL) R8 - 1 Bottle 1x Stop Solution (6 mL) R9 - 1 Vial of Positive Control (0.7 mL) R10 - 2 Adhesive Plate Sealers R11 - User Manual Instructions
Regulatory Information RUO
Technical Notes From one to 88 samples can be run simultaneously or individually (12 breakable strips of 8 wells per 96 well microplate / per kit)
Application Testing Sperm Vitality in samples such as Ejaculates, semen in extenders and Isolated spermatozoa
Articles & Publications Dordas-Perpinyà M, Sergeant N, Ruelle I, Bruyas JF, Charreaux C, Michaud S, Carracedo S, Catalán J, Miró J, Delehedde M and Briand-Amirat L (2022) ProAKAP4 semen concentrations as a valuable marker protein of post-thawed semen quality and bull fertility: a retrospective study. Veterinary Sciences. Vol. 9 :224. https://doi.org/10.3390/vetsci9050224 Marques de Almeida AB, Megumy Tsunokawa HidalgoM, Zito de Moraes FL, Corsi Trautwein LG, Schnitzer J, Dos Santos Silva LA, Rizzoto G, Pinheiro Ferreira JC and Mello Martins MI (2022) The proAKAP4 concentra-tions in Nellore bulls' sperm and its relations to FTAI conception rates results. Rev Bras Reprod Anim. Vol. 46(2)178-179. Kowalczyk A, Gałeska E and Bubel A (2022) The concentration of proAKAP4 and other indicators of cryopotential of spermatozoa cryopreserved in extender with holothuroidea extract addition. Animals. Vol. 12:521. https:// doi.org/10.3390/ani12040521 Carracedo S, Briand-Amirat L, Dordas-Perpinyà M, Ramos Escuredo Y, Delcombel R, Sergeant N and Dele-hedde M (2022) ProAKAP4 protein marker: towards a functional approach to male fertility. Animal Reproduc-tion Science (in Press). Bastan I and Akcay E (2021) Quality assessment of frozen bull semen with the precursor A-kinase anchor protein 4 biomarker. Andrologia e14164. https:// doi.org/10.1111/and.14164 Ruelle I, Sergeant N, Bencharif D, Charreaux F, Thorin C, Michaud S, Dordas-Perpinyà M, Jouy N, Audry S, Maurage CA, Delehedde M and Briand-Amirat L (2020) ProAKAP4 concentrations in semen as a predictive tool of bull fertility: A preliminary study. Reproduction, Fertility and Development. 32(1):145. Carracedo S, Loyens A, Eddarkaoui S, Serteyn D, Malo C, Skidmore L, Briand-Amirat L, Barbotin AL, Maurage CA, Delehedde M and Sergeant N (2020) The sperm specific proAKAP4 polypeptide exhibited conserved functions, localizations and metabolism among mammals. Animal Reproduction Science. Vol. 220 :106448 Sergeant N, Carracedo S, Blommaert D, Aubry S, Jouy N, Lejeune JP, Franck T, Maurage CA, Serteyn D, Buée L and Delehedde M (2020) Proteolysis of proAKAP4 in semen as a regulatory sensor of sperm quality and functionality. Andrology. Vol. 8:44-45. Sergeant N, Briand-Amirat L, Bencharif D and Delehedde M (2019) The sperm specific protein proAKAP4 as an innovative marker to evaluate sperm quality and fertility. Journal of Dairy & Veterinary Sciences. Vol. 11:01-19. Fu Q, Pan L, Huang D, Wang Z, Hou Z and Zhang M (2019) Proteomic profiles of buffalo spermatozoa and seminal plasma. Theriogenology. Vol. 134:74-82. Ruelle I, Charreaux F, Bencharif D, Thorin C, Michaud S, Schmitt E, Sergeant N, Briand-Amirad L and Delehedde M (2019) Assessment of the sperm specific protein proAKAP4 as a marker to evaluate sperm quality and fertility in Holstein bulls. Rev. Bras. Reprod. Anim. Vol.43(2):472. Peddinti D, Nanduri B, Kaya A, Feugang JM, Burgess SC and Memili E (2008) Comprehensive proteomic analysis of bovine spermatozoa of varying fertility rates and identification of biomarkers associated with fertility. BMC Systems Biology. Vol. (2)19:1-13.

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Bull Sperm Vitality Assay Kit (ELISA)

  • Brand: 4BioDx
  • Product Code: 4BioDx-4VDX-18K4
  • Availability: Pre-Order

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Tags: proAKAP4, sperm, semen