Sentinel assays play a crucial role in laboratory animal health monitoring by providing a proactive approach to detect infectious agents and maintain the health of animal colonies. These Sentinel assays are designed to monitor the health status of laboratory animals by using sentinel animals—typically rodents—exposed to the same environment as the study animals. This method allows for the early detection of pathogens that could compromise animal health and affect research outcomes.

Methodology: In sentinel programs, sentinel animals are housed in the same room as the experimental animals. They are periodically tested for the presence of infectious agents through various methods, including serology, PCR, and fecal testing. Common practices involve using soiled bedding from the study animals to expose sentinels to potential pathogens.

Pathogen Detection: Sentinel assays can identify a wide range of pathogens, including viruses, bacteria, and parasites. Specific agents tested may include mouse hepatitis virus (MHV), Sendai virus, and lymphocytic choriomeningitis virus (LCMV). The results help assess the effectiveness of barrier systems and overall animal health.

Compliance: Sentinel assays are essential for maintaining compliance with animal welfare regulations and ensuring that research protocols are not adversely affected by undetected pathogens. They help uphold the integrity of research findings by ensuring that animal health is closely monitored.

Sentinel assays are particularly valuable in toxicology studies and other research involving animal models. By monitoring the health of sentinel animals, researchers can gain insights into the overall health status of the colony and make informed decisions regarding animal care and use.